Comparative study between standard pyocyanin and extracted pyocyanin and synergistic with antibiotics against multidrug bacteria isolated from different clinical samples

Background: Multidrug Resistance forms in Gram positive and negative bacteria are difficult to treat, medications be taken with caution may unsuccessful. The emergence of treat MDR Gram-negative Gram-positive Infections bacterial is still a major issue. Aims: goal this study reliable develop new agents or treatment methods that capable overcoming drug resistance these organisms. Methods: 536(85.3%) samples from out inpatients who admitted Al Diwaniyah Teaching Hospital city were collected various site infection , 92 (46%) Pseudomonas aeruginosa investigated by qualified consulting doctor. After cultivation special media (MacConkey agar Nutrient agar) detect the identified using Vitec 2 compact system, Pyocyanin was extracted standard Principal antimicrobial compounds present pyocyanin HPLC . Other pathogenic obtained for exposure like Escherichia coli K. pneumoniae S. aureus laboratories These diagnosis confirmed Vitek system Serratia marcescens Proteus mirabilis sputum patients co- Covid 19.